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GeneKam Biotechnology AG PCR檢測(cè)試劑盒-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-19      瀏覽次數(shù):191

GeneKam Biotechnology AG  PCR檢測(cè)試劑盒


Genekam Biotechnology AG公司簡(jiǎn)介

Genekam Biotechnology AG成立于1999年,從2004年至2015年已向全球70個(gè)國(guó)家提供PCR檢測(cè)試劑盒。

Genekam Biotechnology AG公司技術(shù)領(lǐng)域

Genekam檢測(cè)產(chǎn)品涵蓋流感病毒、中東呼吸綜合征病毒(MERS)、丙型肝炎病毒、登革熱、皰疹病毒、分枝桿菌、衣原體、支原體、沙門(mén)氏菌等食品病原體等。

Genekam致力于為大流行性病毒的預(yù)防、檢測(cè)和治療提供解決方案。

細(xì)胞培養(yǎng)基中2-3%的胎牛血清足以促進(jìn)人單核細(xì)胞培養(yǎng)物的發(fā)育和生長(zhǎng)

目的:通常的做法是,10-20%的胎牛血清(FCS)被用于細(xì)胞培養(yǎng)基中,用于人單核細(xì)胞以及其他細(xì)胞培養(yǎng)物的發(fā)育和維持。FCS是通過(guò)涉及動(dòng)物的痛苦過(guò)程產(chǎn)生的,是主要的成本因素之一。FCS數(shù)量的減少可以減少其不道德行為,并大大降低成本。

材料與方法: 因此,將2-3%FCS10%FCS用于開(kāi)發(fā)不同的單核細(xì)胞培養(yǎng)物進(jìn)行比較,并測(cè)試了不同的參數(shù),如形態(tài)學(xué)、抗體的產(chǎn)生、用微型柱和磁珠分離DNA/RNA細(xì)胞以及人類(lèi)內(nèi)部對(duì)照的存在與實(shí)時(shí)PCR

結(jié)果:結(jié)果發(fā)現(xiàn),在上述參數(shù)中,2-3%FCS10%FCS的培養(yǎng)基之間沒(méi)有差異。開(kāi)發(fā)了一百多種這樣的細(xì)胞培養(yǎng)物,其中許多細(xì)胞培養(yǎng)物維持了 3 年以上。

結(jié)論:2-3%FCS足以長(zhǎng)期開(kāi)發(fā)和維持人類(lèi)單核細(xì)胞培養(yǎng)物。

 

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